This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: Patent application number 12/543,046 titled "Amplification of Single Viral Genomes". Department of Energy, Cooperative Agreement No. Craig Venter Institute and the Office of Science (BER), U.S. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This research was supported by the J. Received: OctoAccepted: FebruPublished: March 23, 2011Ĭopyright: © 2011 Allen et al. PLoS ONE 6(3):Įditor: Jean-Pierre Vartanian, Institut Pasteur, France The isolation and genome sequencing of uncultivated viruses using Single Virus Genomics approaches will enable researchers to address questions about viral diversity, evolution, adaptation and ecology that were previously unattainable.Ĭitation: Allen LZ, Ishoey T, Novotny MA, McLean JS, Lasken RS, Williamson SJ (2011) Single Virus Genomics: A New Tool for Virus Discovery. The complete lambda phage genome was recovered with an average depth of coverage of approximately 437X. Genome amplification was then achieved in situ via multiple displacement amplification (MDA). coli bacteriophages lambda and T4, were sorted using flow cytometric methods and subsequently immobilized in an agarose matrix. A mixed assemblage comprised of two known viruses E. We report here on a new approach called ‘Single Virus Genomics’, which enabled the isolation and complete genome sequencing of the first single virus particle. However, viral genomics continues to suffer due to difficulties related to the isolation and characterization of uncultivated viruses. Whole genome amplification and sequencing of single microbial cells has significantly influenced genomics and microbial ecology by facilitating direct recovery of reference genome data.
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